DNA
Optimized

Part:BBa_K4043008

Designed by: Jie Wang   Group: iGEM21_United_Shanghai_HS   (2021-09-25)


In-frame expression genes of PETase

ISF6_4831 has a high sequence similarity with the PET hydrolyzing horn, but its hydrolytic activity and selectivity for PET are significantly higher than other PET hydrolyzing enzymes. The enzyme ISF6_4831 required for the degradation of PET plastic by Osaka bacteria was optimized, and a redesigned enzyme with significantly enhanced stability was obtained.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 361
    Illegal SapI site found at 616


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Categories
Parameters
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